Modified gram positive bacteria and uses thereof
AssigneeIntrexon Actobiotics NV
InventorsLothar Steidler, Karolien Van Huynegem, Klaas Vandenbroucke
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Abstract
The present invention relates to gram positive bacteria with increased stress resistance and/or improved storage characteristics. In particular, the invention relates to gram positive bacterium which accumulate intracellular trehalose. The gram positive bacterium according to the invention lack cellobiose-specific PTS system IIC component (PtcC) activity. The gram positive bacterium may further lack trehalose 6-phosphate phosphorylase (TrePP) activity. The gram positive bacterium may further overexpress trehalose transporters. The invention further relates to compositions comprising such gram positive bacterium as well as methods and uses thereof.
Claims22
1-28. (canceled)
A composition comprising a Gram-positive bacterium, wherein the Gram-positive bacterium lacks PtcC activity, wherein the Gram-positive bacterium contains a functional heterologous trehalose-6-phosphate phosphatase gene, wherein the Gram-positive bacterium further (a) constitutively overexpresses a gene encoding a trehalose transporter, or (b) lacks trehalose 6-phosphate phosphorylase (TrePP) activity, and wherein the composition is a food additive, a probiotic composition, or a starter culture.
Dependencies are inferred from the claim text.