Inducible promoter for gene expression and synthetic biology
AssigneesInstitut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement, Universite Libre de Bruxelles, Universite De Liege
InventorsJean-Marc NICAUD, Marion TRASSAERT, Stephane THOMAS, Patrick FICKERS, Marie VANDERMIES, Frederic CARLY
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Abstract
The invention is related to an inducible promoter for improved and regulated gene expression, useful in synthetic biology and metabolic engineering. In particular, the present invention relates to a nucleotide sequence comprising the regulatory regions of an erythritol- and erythrulose-inducible promoter in yeast and uses thereof in an expression system thus allowing an improved and regulated gene expression and production of gene product.
Claims19
A nucleotide sequence comprising at least one sequence according to formula (I) and/or at least one sequence according to formula (II), wherein:
- formula (I) is
- CGGNNX1CNNNANNX2GNNAAGNCG (I)
- with N being any nucleotide
- with X1 and X2 being any nucleotide or nothing, as set forth in anyone of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3 and SEQ ID NO:
- 4
- formula (II) is
- ANTTNNNTTTCCNNATNNGG (II)
- with N being any nucleotide, as set forth in SEQ ID NO:
- 5.
Dependencies are inferred from the claim text.