Virus causing respiratory tract illness in susceptible mammals
AssigneeErasmus University Medical Center Rotterdam
InventorsJan Cornelius DE JONG, Ronaldus Adrianus Maria FOUCHIER, Bernadetta Gerarda VAN DEN HOOGEN, Albertus D.M.E. OSTERHAUS, Jan GROEN
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Abstract
The invention relates to the field of virology. The invention provides an isolated essentially mammalian negative-sense single-stranded RNA virus (MPV) within the subfamily Pneumovirinae of the family Paramyxoviridae and identifiable as phylogenetically corresponding to the genus Metapneumovirus and components thereof.
Claims13
A kit for determining the presence of metapneumovirus (MPV) in a mammalian subject, the kit comprising:
- a DNA probe of at least 10 nucleotides that specifically hybridizes to a target polynucleotide,
- wherein the target polynucleotide comprises a sequence encoding a polypeptide that is at least 90% identical to SEQ ID NO:28, SEQ ID NO:32, or SEQ ID NO:39, or the complement of the sequence of the sequence encoding the polypeptide, and
- wherein the DNA probe does not specifically hybridize to a polynucleotide from avian pneumovirus (APV).
A method for detecting a human metapneumovirus infection in a mammal, the method comprising:
- detecting, in a sample from the mammal, the presence of a component of human metapneumovirus by reacting the sample with a DNA probe of at least 10 nucleotides in length that specifically hybridizes to a target polynucleotide,
- wherein the target polynucleotide comprises a sequence encoding a polypeptide that is at least 90% identical to SEQ ID NO:28, SEQ ID NO:32, or SEQ ID NO:39 or the complement of the sequence encoding the polypeptide, and
- wherein the DNA probe does not specifically hybridize to a polynucleotide from avian pneumovirus (APV).
The method according to claim 32, further comprising performing an nucleic acid amplification reaction on a sample derived from the mammalian subject utilizing a set of primers for the amplification of at least a portion of the F gene of human metapneumovirus to create an amplified product.
Dependencies are inferred from the claim text.