Mineral coated microparticles for gene delivery in chronic wound therapy
AssigneeWisconsin Alumni Research Foundation
InventorsWilliam L. MURPHY, Andrew Salim KHALIL, Xiaohua YU
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Abstract
Disclosed are formulations for providing a therapeutic bioactive polypeptide to injured tissue. Formulations include mineral coated microparticles wherein a polynucleotide is adsorbed to the mineral layer. Other formulations include a carrier including mineral coated microparticles wherein mineral coated microparticles include a polynucleotide. Also disclosed are methods for sustained delivery of a bioactive polypeptide and methods for treating chronic wounds using a formulation for providing sustained delivery of the bioactive peptide.
Claims26
A method of repairing injured tissue in a subject in need thereof, the method comprising:
- contacting a microparticle comprising a mineral layer with a polynucleotide, wherein the polynucleotide is adsorbed to the mineral layer;
- and
- contacting the microparticle with the injured tissue.
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A method for sustained delivery of bioactive polypeptides, the method comprising:
- contacting a mineral coated microparticle comprising a mineral layer with a polynucleotide adsorbed thereto with at least one cell, wherein, as the coating of the mineral coated microparticle degrades, the microparticle releases a bioactive polypeptide encoded by the polynucleotide to the cell;
- and
- sequestering the bioactive polypeptide by the mineral coated microparticle.
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Dependencies are inferred from the claim text.