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US 2020/0138351 A1Published application
Method for Measuring Fluorescence in Ocular Tissue
AssigneeCognoptix, Inc.
InventorsCharles Kerbage, Paul D. Hartung
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Abstract
A method is provided for ophthalmic measurements, wherein the amount of a fluorophore is detected in ocular tissue and the obtained fluorescence signals are normalized by performing a ratio.
Claims1
A method for measuring the amount of a fluorophore in ocular tissue, the method comprising the following steps:
- a) contacting the ocular tissue with a first fluorophore that specifically binds to a protein;
- b) illuminating the ocular tissue with a light source suitable to elicit fluorescence of the first fluorophore and suitable to elicit fluorescence of a second fluorophore, which is used as a reference;
- c) determining a first light signal intensity for a selected lifetime value (τ1) or a lifetime interval (dt1) of the fluorescence emitted by the first fluorophore and a second light signal intensity for a selected lifetime value (τ2) or a lifetime interval (dt2) of the second fluorophore, wherein the first and second light signals are derived from the same region in the eye;
- d) determining a ratio (r) of the first signal intensity to the second signal intensity, and
- e) using the ratio (r) of the first to the second signal intensity for normalization of the determined light signal intensities.
2 more elements
Dependencies are inferred from the claim text.