Method for direct reprogramming of urine cells into keratinocyte stem cells and method for preparing composition for promoting skin regeneration using reprogrammed keratinocyte stem cells
AssigneeStemlab Inc.
InventorsEun Kyoung JUN, Jie ZHENG, Phil Jn KANG, DaRyeon SON, Ji-Hoon JANG, Won Jun HONG, Jung-Hyun PARK, Gyu Man PARK, In Yong KIM
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Abstract
The present invention relates to a method for inducing reprogramming of urine cells into keratinocyte stem cells by introducing reprogramming factors Bmi1 and dNP63a, and a composition for promoting skin regeneration which includes an induced reprogrammed keratinocyte stem cell conditioned medium as an active ingredient.
Claims19
A composition for direct reprogramming urine cells into keratinocyte stem cells, the composition comprising:
- (i) a Bmi1 protein, or a coding sequence of Bmi1 protein;
- and
- (ii) a dNP63a protein, or a coding sequence of dNP63a protein.
A method for direct reprogramming urine cells into keratinocyte stem cells, the method comprising:
- (a) Isolating urine cells from urine and culturing the urine cells;
- (b) Transducing cording sequence of Bmi1 and dNP63a into the isolated and cultured urine cells;
- (c) Culturing the transduced urine cells under keratinocyte stem cell culture conditions;
- and
- (d) Selecting a reprogrammed keratinocyte stem cell line having characteristics similar to keratinocyte stem cells from the urine cells which have been cultured.
A method for preparing a reprogrammed keratinocyte stem cell conditioned medium or a culture solution thereof from urine cells, the method comprising:
- (a) Isolating urine cells from urine and culturing the urine cells;
- (b) Transducing cording sequence of Bmi1 and dNP63a into the isolated and cultured urine cells;
- (c) Culturing the transduced urine cells under keratinocyte stem cell culture conditions;
- and
- (d) Selecting a reprogrammed keratinocyte stem cell line having characteristics similar to keratinocyte stem cells from the urine cells which have been cultured;
- and
- (e) Acquiring a conditioned medium or a culture solution thereof by culturing the selected reprogrammed keratinocyte stem cells in a serum-free medium.
Dependencies are inferred from the claim text.