FDCA-decarboxylating monooxygenase-deficient host cells for producing FDCA
AssigneePurac Biochem B.V.
InventorsJohannes Adrianus Maria de Bont, Harald Johan Ruijssenaars, Jan Werij
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Abstract
The invention relates to fungal cells for the production of FDCA. The fungal cell has genetic modification that reduces specific 2,5-furandicarboxylic acid (FDCA) decarboxylating monooxygenase activity in the cell, as compared to a corresponding parent cell lacking the genetic modification. The fungal cell can further be genetically modified to increase the cell's ability to oxidize furanic aldehydes to the corresponding furanic carboxylic acids. The invention also relates to a process for the production of 2,5-furan-dicarboxylic acid (FDCA) wherein the cells of the invention are used for oxidation of a furanic precursors of FDCA to FDCA.
Claims18
A fungal cell comprising a genetic modification that reduces specific 2,5-furandicarboxylic acid (FDCA) decarboxylating monooxygenase activity in the cell, as compared to a corresponding parent cell lacking the genetic modification, wherein the genetic modification eliminates the expression of an endogenous gene encoding an FDCA decarboxylating monooxygenase by deletion of at least a part of at least one of the promoter and the coding sequence of the gene.
A fungal cell having the ability to oxidize HMF to FDCA and comprising a genetic modification that increases expression of a nucleotide sequence encoding a polypeptide that transports at least one furanic compound, which polypeptide preferably comprises an amino acid sequence that has at least 45% sequence identity with the amino acid sequence of at least one of SEQ ID NO.'s:
- 9 and 10.
-16. (canceled)
Dependencies are inferred from the claim text.