Methods and Systems to Produce Lignin-Modifying Enzymes and Uses Thereof
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsNikita A. Khlystov, Elizabeth Sattely
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Abstract
Embodiments of the invention are generally directed to lignin-modifying enzymes and systems and methods of their manufacture. In many embodiments, the lignin-modifying enzymes are lignin-degrading enzymes capable of breaking down lignin into component parts that are usable for other purposes. Several embodiments are directed to systems for producing lignin-modifying enzymes in vivo, including in yeast and/or plant species, and certain embodiments are directed to methods of creating these systems, including transfecting the species to produce lignin-modifying enzymes.
Claims20
A vehicle for gene expression comprising an organism capable of expressing a gene transformed with an expression vector containing a lignin-modifying enzyme.
A method to produce lignin-modifying enzymes comprising:
- obtaining an expression vector containing a lignin-modifying enzyme;
- transforming an organism with the expression vector;
- allowing the organism to express the lignin-modifying enzyme contained within the expression vector;
- and
- extracting the lignin-modifying enzyme.
Dependencies are inferred from the claim text.