Systems and methods for single-cell rna-seq data analysis

Abstract

Disclosed are computer-implemented methods, systems, and media for clustering cells using gene differential expression of single cells. In an aspect, a method may comprise: mapping RNA-Seq data of a plurality of cells onto a sphere (e.g., a hypersphere); calculating a plurality of distances, each of which is associated with an angle between two different cells mapped onto the sphere; clustering the plurality of cells into two clusters based on the plurality of distances; evaluating each of the two clusters using a pre-determined stopping criterion; and repeating the clustering and evaluating on each of the two clusters until the pre-determined stopping criterion or a second stopping criterion is met.

Dependencies are inferred from the claim text.