Method of detecting and/or identifying adeno-associated virus (aav) sequences and isolating novel sequences identified thereby
AssigneeThe Trustees of the University of Pennsylvania
InventorsGuangping Gao, James M. Wilson, Mauricio R. Alvira
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Abstract
Adeno-associated virus rh.20 sequences, vectors containing same, and methods of use are provided.
Claims21
A recombinant adeno-associated virus (AAV) comprising an AAV capsid comprising vp1 proteins having the sequence of amino acids 1 to 738 or a sequence at least 95% identical to the full length of amino acids 1 to 738 of SEQ ID NO:
- 85, AAV vp2 proteins, and AAV vp3 proteins, wherein the recombinant AAV further comprises, packaged within the capsid, a nucleic acid molecule comprising at least one AAV inverted terminal repeat (ITR), and a non-AAV nucleic acid sequence encoding a gene product operably linked to sequences which direct expression of the gene product in a host cell, wherein the gene product is a dystrophin protein.
A recombinant adeno-associated virus (AAV) having an AAV capsid comprising AAV vp1 proteins, AAV vp2 proteins having a sequence of amino acids 138 to 738 of SEQ ID NO:85 or a sequence at least 95% identical to the full-length of amino acids 138 to 738 of SEQ ID NO:
- 85, and AAV vp3 proteins, wherein the recombinant AAV further comprises, packaged within the capsid, a nucleic acid molecule comprising at least one AAV inverted terminal repeat (ITR) and a non-AAV nucleic acid sequence encoding a gene product operably linked to sequences which direct expression of the gene product in a host cell, wherein the gene product is a dystrophin protein.
A recombinant adeno-associated virus (AAV) having an AAV capsid comprising AAV vp1 proteins, AAV vp2 proteins, and AAV vp3 proteins having a sequence of amino acids 204 to 738 of SEQ ID NO:85 or a sequence at least 95% identical to the full-length of amino acids 204 to 738 of SEQ ID NO:
- 85, wherein the recombinant AAV further comprises, packaged within the capsid, a nucleic acid molecule comprising at least one AAV inverted terminal repeat (ITR) and a non-AAV nucleic acid sequence encoding a gene product operably linked to sequences which direct expression of the gene product in a host cell, wherein the gene product is a dystrophin protein.
Dependencies are inferred from the claim text.