Enzymes for Glycan Analysis
AssigneeGenovis AB
InventorsFredrik Leo, Rolf Lood, Stephan Bjork, Malin Mejare, Fredrik Olsson
Life of this patent
Estimating the term…
Abstract
The present invention relates to enzymes and combinations thereof useful for studying glycoproteins, and corresponding methods of use. In particular, the invention relates to a sialidase composition comprising an additional protease and/or glycosidase, preferably an O-glycoprotein-specific endoprotease and/or an O-glycosidase.
Claims12
A composition comprising a first sialidase which is independently selected from:
- (a) a polypeptide comprising or consisting of an amino acid sequence of SEQ ID NO:
- 2;
- (b) a polypeptide comprising or consisting of an amino acid sequence which is at least 85% identical to the amino acid sequence of SEQ ID NO:
- 2 or
- (c) a polypeptide comprising or consisting of an amino acid sequence which is a fragment of the sequence of SEQ ID NO:
- 2 or a fragment of an amino acid which is 85% identical to the amino acid sequence of SEQ ID NO:
- 2;
- optionally wherein said first sialidase includes an additional methionine at the N terminus and/or a histidine tag at the C terminus, which tag may be joined to the C terminus by a linker.
The composition according to any one of the preceding claims, which comprises the sialidase consisting of the amino acid sequence of SEQ ID NO:
- 3 and the sialidase consisting of the amino acid sequence of SEQ ID NO:
- 6, preferably in a 1:1 ratio.
The composition according to any one of the preceding claims, wherein the first sialidase and/or the second sialidase are present in highly purified or isolated form;
- and/or wherein no more than two polypeptides in the composition are sialidases obtained from Akkermansia muciniphila.
The composition according to any one of the preceding claims, which additionally comprises a glycosidase and/or a protease, which is optionally present in highly purified or isolated form.
A method for modifying a glycoprotein comprising contacting a sample containing the glycoprotein with a composition according to any one of the preceding claims, optionally wherein the resulting products are analysed.
Dependencies are inferred from the claim text.