Bioreactor module, a bioreactor system and methods for thick tissue seeding and cultivation in an hierarchical organization and physiological mimicking conditions
AssigneesNanyang Technological University, Technion Research & Development Foundation Limited
InventorsMarcelle MACHLUF, Udi SARIG, Maskit GVIRTZ, Subramanian VENKATRAMAN
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Abstract
According to various embodiments, there is provided a bioreactor module including a container; a holder removably receivable in the container, the holder adapted to hold a scaffold containing an inherent vascular network; an inlet connectable to a vessel of the inherent vascular network of the scaffold; an inflatable device disposed substantially near a base of the container, the inflatable device having a conduit extending through a wall of the container; and a pair of electrodes attached to opposing walls of the container.
Claims20
A bioreactor module comprising:
- a container;
- a holder removably receivable in the container, the holder including any one of a clamping mechanism, a gripping mechanism, a hook, or an attachment mechanism configured to hold a scaffold containing an inherent vascular network;
- an inlet connectable to a vessel of the inherent vascular network of the scaffold;
- an inflatable device disposed within the container, the inflatable device having a conduit extending through a wall of the container;
- and
- a pair of electrodes attached to opposing walls of the container.
A bioreactor system comprising a bioreactor module that includes:
- a container;
- a holder removably receivable in the container, the holder comprises any one of a clamping mechanism, a gripping mechanism, a hook, or an attachment mechanism so as to hold a scaffold containing an inherent vascular network;
- an inlet connectable to a vessel of the inherent vascular network of the scaffold;
- an inflatable device disposed within the container, the inflatable device having a conduit extending through a wall of the container;
- and
- a pair of electrodes attached to opposing walls of the container.
An in-vitro method for tissue cultivation, comprising:
- seeding an interior of a vessel of an inherent vascular network of a scaffold with a first cell type;
- seeding an exterior surface of the scaffold with a second cell type;
- and
- perfusing through the inherent vascular network of the scaffold with culture medium to facilitate compartmentalized co-cultivation of the first cell type and the second cell type in different niches of the tissue.
An in-vitro method for tissue cultivation, comprising:
- seeding a surface of a scaffold with a predetermined cell type;
- and
- perfusing the scaffold from an opposite surface of the scaffold through the scaffold and towards the seeded surface with culture medium to provide flow of nutrients and oxygen through the scaffold to create a nutrient/oxygen gradient between the opposite surface and the seeded surface of the scaffold to cause migratory diffusion induced penetration of cells towards the opposite surface.
Dependencies are inferred from the claim text.