Loading patent…
AssigneesArcticzymes AS, Universitetet I Tromsø—Norges Arktiske Universitet
InventorsTerese SOLSTAD, Elisabeth Lill ANDREASSEN, Marit Sjo LORENTZEN, Olav LANES, Morten ELDE, Atle Noralf LARSEN, Yvonne PIOTROWSKI, Nils Peder WILLASSEN
Estimating the term…
The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55° C. for 10 minutes in a buffer consisting of 10 mM Tris-HCl, pH 8.5 at 25° C., 50 mM KCl and 5 mM MgCl2; (ii) is substantially specific for single stranded DNA; and (iii) has a 3′-5′ exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used. The invention still further provides nucleic acids encoding said exonuclease or an enzymatically active fragment thereof and kits or compositions comprising the same.
A method of nucleic acid sequence analysis, said method comprising a step of sample preparation prior to the analysis step(s), said step of sample preparation comprising contacting the sample to be analysed with an exonuclease or enzymatically active fragment thereof under conditions which permit the digestion of at least a portion of any single stranded DNA present in the sample and optionally then heating the mixture to inactivate said exonuclease or enzymatically active fragment thereof,
Dependencies are inferred from the claim text.