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US 2020/0071732 A1Published application
Production of Cannabinoids in Yeast
AssigneeLibrede Inc.
InventorsJason L. Poulos, Anthony N. Farina
Life of this patent
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Abstract
Exemplary embodiments provided herein include genetically engineering microorganisms, such as yeast or bacteria, to produce cannabinoids by inserting genes that produce the appropriate enzymes for the metabolic production of a desired compound.
Claims15
A method for making Cannabigerovarinic Acid in yeast, the method comprising:
- converting glucose to malonyl-CoA with a first nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.124 expressing MatB;
- converting malonyl-CoA to Acetoacetyl-CoA with a second nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO. 118 expressing MCT1;
- converting Acetoacetyl-CoA to 3-Hydroxybutyryl-CoA with a third nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO. 119 expressing 3-hydroxybutyryl-CoA dehydrogenase (Hbd);
- converting 3-Hydroxybutyryl-CoA to Crotonyl-CoA with a fourth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO. 120 expressing crotonase (Crt);
- converting Crotonyl-CoA to Butyryl-CoA with a fifth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.123 expressing trans-enoyl-CoA reducatase (Ter);
- converting Butyryl-CoA to divarinic acid with a sixth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.B expressing Polyketide synthase and with a seventh nucleotide sequence of SEQ. ID. NO.E expressing olivetolic acid cyclase;
- SEQ ID NO B where B=60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72;
- Where SEQ ID NO E=73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84;
- converting divarinic acid to Cannabigerovarinic Acid with an eighth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.A expressing an Aromatic prenyltransferase;
- and SEQ ID NO A where A=30, 31, 32, 36, 37, 38, 42, 43, 44, 48, 49, 50, 54, 55, 56, 33, 34, 35, 39, 40, 41, 45, 46, 47, 51, 52, 53, 57, 58, 59.
7 more elements
A method for making cannabigerolic acid in yeast, the method comprising:
- converting glucose to malonyl-CoA with a first nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.124 expressing MatB;
- converting malonyl-CoA to Acetoacetyl-CoA with a second nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.118 expressing MCT1;
- converting Acetoacetyl-CoA to 3-Hydroxybutyryl-CoA with a third nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO. 119 expressing 3-hydroxybutyryl-CoA dehydrogenase (Hbd);
- converting 3-Hydroxybutyryl-CoA to Crotonyl-CoA with a fourth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.120 expressing crotonase (Crt);
- converting Crotonyl-CoA to Butyryl-CoA with a fifth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.123 expressing trans-enoyl-CoA reducatase (Ter);
- converting Butyryl-CoA to 3-Ketonhexanoyl-CoA with a sixth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.122 expressing β-ketothiolase (Bktb);
- converting 3-Ketonhexanoyl-CoA to 3-Hydroxyhexanoyl-CoA with a seventh nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.119 expressing Hbd;
- converting 3-Hydroxyhexanoyl-CoA to Trans-hexa-2-enoyl-CoA with an eighth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.120 expressing Crt;
- converting Trans-hexa-2-enoyl-CoA to Hexanoyl-CoA with an ninth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.123 expressing Ter;
- converting Hexanoyl-CoA to Olivetolic acid with a tenth nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO. B expressing Polyketide synthase and with an eleventh nucleotide sequence of SEQ. ID. NO. E expressing cyclase;
- SEQ ID NO B where B=60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72;
- where SEQ ID NO E=73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84;
- converting Olivetolic acid to cannabigerolic acid with an eleventh nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO.A expressing an Aromatic prenyltransferase, SEQ ID NO A where A=30, 31, 32, 36, 37, 38, 42, 43, 44, 48, 49, 50, 54, 55, 56, 33, 34, 35, 39, 40, 41, 45, 46, 47, 51, 52, 53, 57, 58, 59.
10 more elements
Dependencies are inferred from the claim text.