Disposable electrochemical sensing strips and associated methods

The electrochemical sensing device for measuring the content of ions in a biological fluid sample comprising:

• a first half cell provided with a first ion-selective electrode made of a first ion-selective membrane and a first conductive support;
• a second half cell provided with a second ion-selective electrode made of a second ion-selective membrane a second conductive support;
• a salt bridge connecting first ion-selective membrane and the second ion-selective membrane;
• means for bringing a biological fluid sample in contact with the salt bridge in the vicinity of the second ion-selective membrane;
• wherein the first and second membranes are selective to the same ions, and which comprises a first calibration volume with a known concentration of the ions to which the membranes are selective, the calibration volume being placed in contact with the salt bridge in the vicinity of the first ion-selective membrane, the salt bridge being filled with a known concentration of the ions to which the membranes are selective, and which comprises a diffusion limiter between the calibration volume and the salt bridge, such that a voltage can be measured between the first electrode and the second electrode that allows calibrating the electrochemical sensing device when the diffusion limiter is removed, and then measuring the ion-content of the biological fluid sample.

An electrochemical sensing device for measuring the content of ions in biological fluid samples comprising:

• a first half cell provided with a first ion-selective electrode made of a first ion-selective membrane and a first conductive support, and a first volume in contact with the first ion-selective membrane;
• a second half cell provided with a second ion-selective electrode made of a second ion-selective membrane and a second conductive support, and a second volume in contact with the second ion-selective membrane;
• a salt bridge connecting the first volume and the second volume;
• and
• means for bringing a biological fluid sample in contact with the second volume,
• wherein the salt bridge comprises a diffusion limiter, which allows opening the salt bridge when it is removed, and wherein the first volume, the second volume and the salt bridge are a hydrogel.