Device for cell spraying

Device for controlled spraying of biological compatible liquids, biological compatible suspensions, and biological cell suspensions including stem cell suspensions, modified stem cell suspensions, cell suspensions for wound regeneration, cell suspensions for dermal cells, cell suspensions for epidermal cells, cell suspensions for dermal and epidermal cells,

• providing continuous spray application over a range of 0.5-10 Minutes, preferably 1-2 Minutes in a single shot and/or in several shots, while generating liquid/suspension drops ranging between 10-500 Micrometer in size, exhibiting a hollow needle to deposit liquids/suspensions, which is
• connected at the outlet of a biocompatible container delivering a liquid/suspension stream towards the tip of the needle,
• injected with the tip into a biocompatible tube delivering a gas and/or air stream in direction to the end of the tube where the tip of the needle is positioned, whereas
• the needle exhibits an inner lumen of 25 G to 34 G, preferably of 27 G to 30 G, and
• the needle is injected into the tube in a way that the tip of the needle ends within +/−3 Millimeter, preferably +/−0.2 Millimeter at the end of the tube,
• the tip of the needle runs within the tube at least 2-100 Millimeter, preferably 4-20 Millimeter in parallel to the tube so that the needle tip is centered in the end of the tube lumen,
• the tube exhibits an inner diameter of 1.5-0.2 Millimeter, preferably of 0.8-0.6 Millimeter,
• the tube bends at the point of needle injection with a radius not prohibiting the gas flow to the side in an angle of 1 Degree-170 Degree, preferably 80 Degree-100 Degree, enabling an easy needle injection,
• the gas stream is preferably a gas/air mix stream, preferably a sterile-filtered air stream, whereas the device delivers
• via the needle a liquid/suspension stream of 1-200 mL/min, preferably 5-30 mL/min,
• via the tube a gas stream of 100-4000 mL/min, preferably 200-2000 mL/min, whereas the gas stream is generated by a compressed gas container, or a gas pump, preferably a compressor in the device or in a separate housing attached to the device via a tube,
• preferably sterile-filtered prior to entering the needle, e.g. by a sterile-filter, whereas the liquid/suspension stream is generated by a compressible container, preferably by a syringe with plunger, which is connected to an actuator, including a pneumatic gas- or hydraulic liquid membrane, or an mechanical/electromechanical pusher, which pushes the plunger of the syringe to generate the liquid/suspension flow,
• whereas the device can be presented as a single, hand held mechanically and/or battery operated device, or a device out of at least 2 components with a handheld spray unit and a support unit including electric power supply which are connected via pneumatic/hydraulic/electric lines,
• whereas the above characterized needle/tube I operation parameter configuration produce a homogeneous and continuous spray stream generated by the flow of the liquid medium out of the tip and of the specific gaseous medium out of the tube, which is less cell suspension harmful than the typical cell spray nozzle using openings far below and turbulences far above the presented configuration;
• whereas either all hand held parts of the device are sterilized prior use, or only the parts containing gas and liquid/suspension streams are sterilized prior use, or whereas all liquid/gas transferring parts are delivered separately sterilized and are assembled in a sterile manner immediately prior to use:
• whereas the device can be embodied as a fully disposable device.