Sesquiterpene synthases for production of drimenol and mixtures thereof
AssigneeFirmenich SA
InventorsPan Li, Qi Wang, Xiu-Feng He, Olivier Haefliger
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Abstract
Described herein is a method of producing drimenol and/or drimenol derivatives, the method including contacting at least one polypeptide with farnesyl diphosphate (FPP). The method may be performed in vitro or in vivo. Also described herein are amino acid sequences of polypeptides useful in the methods and nucleic acids encoding the polypeptides described. Also described herein are host cells or organisms genetically modified to express the polypeptides and useful to produce drimenol and/or derivatives of drimenol.
Claims20
A method for producing drimenol or a mixture comprising drimenol comprising:
- a. contacting an acyclic farnesyl diphosphate (FPP) precursor with a polypeptide having sesquiterpene synthase activity, wherein the polypeptide comprises an amino acid sequence having at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO:
- 2 or comprises the amino acid sequence of SEQ ID NO:
- 2, to produce drimenol or a mixture comprising drimenol and one or more terpenes;
- and
- b. optionally isolating the drimenol.
An isolated polypeptide having sesquiterpene synthase activity comprising an amino acid sequence having at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO:
- 2 or comprising the amino acid sequence of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.