Angular leaf spot (pseudomonas) resistance in cucumber
AssigneeRijk Zwaan Zaadteelt En Zaadhandel B.V.
InventorCornelis Van Der Maas
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Abstract
The present invention relates to a cucumber plant (Cucumis sativus L.) that is resistant to Pseudomonas syringae pv. lachrymans, a pathogen that causes angular leaf spot disease in cucumber. The invention further relates to markers linked to the resistance and the use of markers to identify resistant plants. The invention also relates to seed and progeny of such plants and to propagation material derived from such plants and for obtaining such plants. Furthermore, the invention relates to methods for producing, identifying and selecting Pseudomonas resistant cucumber plants, and for producing hybrid plant seed. The invention further relates to the use of a gene and/or QTLs and sequences thereof to identify/select Pseudomonas resistant cucumber plants.
Claims44
A cucumber plant that comprises a mutant allele of the Gibberellin-20-oxidase gene, that confers resistance to Pseudomonas syringae pv. lachrymans to the plant.
A mutant allele of the Gibberellin-20-oxidase gene, comprising a SNP on position 860 of the wild type nucleotide sequence of SEQ ID NO:1.
A mutant Gibberellin-20-oxidase protein, comprising a substitution on position 287 of the wild type amino acid sequence of SEQ ID NO:3.
(canceled)
A method for identifying a cucumber plant showing resistance to Pseudomonas syringae pv. lachrymans comprising:
- a) detecting in a cucumber plant a SNP in the wild type nucleotide sequence of SEQ ID NO:1, thereby identifying a cucumber plant showing resistance to Pseudomonas syringae pv. lachrymans;
- b) optionally performing a phenotypical assay for Pseudomonas syringae pv. lachrymans resistance, to confirm that the cucumber plant is resistant to Pseudomonas syringae pv. lachrymans.
A method for producing a hybrid cucumber seed, wherein a plant grown from such seed shows resistance to Pseudomonas syringae pv. lachrymans, wherein the method comprises crossing a first parent plant with a second parent plant and harvesting the resultant seed, wherein the first parent plant and/or the second parent plant comprises a mutant allele of the Gibberellin-20-oxidase gene, wherein the mutant allele of the Gibberellin-20-oxidase gene confers resistance to Pseudomonas syringae pv. lachrymans in a plant grown from the seed.
A cell of a Pseudomonas syringae pv. lachrymans resistant cucumber plant, which cell comprises a mutant allele of the Gibberellin-20-oxidase gene, comprising a SNP on position 860 of the wild type nucleotide sequence of SEQ ID NO:1.
A tissue culture of a cucumber plant having resistance to Pseudomonas syringae pv. lachrymans, which tissue culture comprises a mutant allele of the Gibberellin-20-oxidase gene, comprising a SNP on position 860 of the wild type nucleotide sequence of SEQ ID NO:1.
A method for producing a cucumber plant having resistance to Pseudomonas syringae pv. lachrymans comprising the steps of—:
- a) introducing mutations in a population of cucumber plants;
- b) selecting a cucumber plant showing resistance to Pseudomonas syringae pv. lachrymans;
- c) verifying if the plant selected under step b) has a mutation in the Gibberellin-20-oxidase gene, wherein the mutation is a SNP in the Gibberelline-20-oxidase gene represented by the wild type nucleotide sequence SEQ ID NO:1, and selecting a plant comprising such a mutation.
- d) growing the plants obtained under step c).
A method for producing a cucumber plant showing resistance to Pseudomonas syringae pv. lachrymans comprising growing a plant from seed which was deposited under deposit number NCIMB 42582 or growing a plant from seed derived thereof.
A method of selecting a cucumber plant for showing resistance to Pseudomonas syringae pv. lachrymans comprising:
- a) assaying genomic nucleic acids of a cucumber plant for the presence of a mutation in the wild type Gibberellin-20-oxidase gene nucleotide sequence of SEQ ID NO:1;
- and
- b) selecting the cucumber plant if said mutation is present.
Dependencies are inferred from the claim text.