Loading patent…
AssigneeOrig3n, Inc.
InventorsRobin Y. Smith, Marcie A. Glicksman
Estimating the term…
Described herein is a managed, indexed repository of hematopoietic stem cell (HSC) lines and/or blood progenitor cell lines derived from induced pluripotent stem cells (iPSCs) (or embryoid bodies formed from iPSCs), wherein each of the HSC lines, blood progenitor cell lines, embryoid bodies, and/or iPSC lines has corresponding data comprising a set of characterized HLA loci, said corresponding data being stored in a searchable database for retrieval of one or more matching physical cell lines upon query. The repository comprises an HLA-indexed bank of iPSCs, embryoid bodies, HSCs, and/or blood progenitor cells for each of a set of HLA types, for identification and provision of allogeneic cell lines suitable for transplantation to reestablish hematopoietic function in patients with damaged, diseased, or otherwise abnormal bone marrow and/or immune system.
A method of querying and retrieving data entries of a HLA-indexed database that match a set of queried human leukocyte antigen (HLA) loci for identification, production, and/or retrieval of hematopoietic stem cells (HSCs) suitable for treatment of a subject, said method comprising the steps of:
The method of claim any one of the preceding claims, wherein the set of characterized HLA loci corresponding to each of the plurality of characterized iPSC lines comprises each of a set of at least 3 given loci, wherein the at least 3 given loci are HLA-A, HLA-B, and HLA-DRB.
The method of any one of the preceding claims, wherein the set of characterized HLA loci corresponding to each of the plurality of characterized iPSC lines comprises at least 9 given loci, wherein the at least 9 given loci are HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5, HLA-DQB1, and HLA-DPB1.
The method of any one of the preceding claims, wherein the set of characterized HLA loci corresponding to each of the plurality of characterized iPSC lines comprises at least 3 given loci selected from the group consisting of HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5, HLA-DQB1, and HLA-DPB1.
The method of any one of the preceding claims, wherein each of the one or more retrieved data entries of the database exactly match or partially match the set of queried HLA loci for the subject.
The method of any one of the preceding claims, further comprising retrieving one or more cells from the physical repository corresponding to the one or more retrieved data entries.
The method of any one of the preceding claims, wherein the data entry corresponding to each of a plurality of characterized induced pluripotent stem cell (iPSC) lines further comprises ABO blood type and the query further comprises ABO blood type, and wherein the retrieving step comprises retrieving, by the processor, one or more data entries, each representative of the iPSC line matching the set of queried HLA loci and the queried ABO blood type.
The method of any one of the preceding claims, wherein the data entry corresponding to each of a plurality of characterized induced pluripotent stem cell (iPSC) lines further comprises RHD blood group and the query further comprises RHD blood group, and wherein the retrieving step comprises retrieving, by the processor, one or more data entries, each representative of the iPSC line matching the queried RHD blood group and the set of queried HLA loci.
The method of any one of the preceding claims, wherein the set of queried HLA loci correspond to the subject in need of an HLA match.
The method of any one of the preceding claims, wherein the set of queried HLA loci is determined by processing and analyzing a biological sample from the subject in need of an HLA match.
The method of any one of the preceding claims, further comprising retrieving characterized cells from the physical repository, wherein the characterized cells correspond to the one or more retrieved data entries matching the set of queried HLA loci.
The method of any one of the preceding claims, further comprising producing blood progenitors and/or HSCs from an iPSC line corresponding to the one or more retrieved data entries matching the set of queried HLA loci.
The method of any one of the preceding claims, further comprising administering blood progenitors and/or HSCs to the subject, wherein said blood progenitors and/or HSCs are derived from an iPSC line corresponding to the one or more retrieved data entries matching the set of queried HLA loci.
The method of any one of the preceding claims, wherein the database comprises a data entry corresponding to each of a plurality of iPSC super donor cell lines, wherein the data entry for each super donor cell line comprises a set of characterized HLA loci corresponding to the super donor cell line.
A system of querying and retrieving data entries of an HLA-indexed database that match a set of queried human leukocyte antigen (HLA) loci for identification, production, and/or retrieval of hematopoietic stem cells (HSCs) suitable for treatment of a subject, the system comprising:
Dependencies are inferred from the claim text.