A computer program comprising instructions which, when the computer program is executed by a computer, cause the computer to determine additional information about a sample on the basis of at least one input record of the sample and on the basis of training information, wherein the input record is specific for an input detection of the sample in accordance with an application technology and the training information is determined according to
- a method for providing an evaluation means for at least one optical application system of a microscope-based application technology,
- wherein the following steps are performed:
- performing an input detection of at least one sample according to the application technology in order to obtain at least one input record of the sample from the input detection,
- performing a target detection of the sample according to a training technology to obtain at least one target record of the sample from the target detection, the training technology being different from the application technology at least in that additional information about the sample is provided,
- training of the evaluation means at least on the basis of the input recording and the target recording in order to obtain training information of the evaluation means,
- wherein various sample positions are automatically detected during input detection or target detection,
- wherein the training information for a continuous relative displacement of the sample for determining the additional information is trained by the detection of the various sample positions,
- wherein the input detection and the target detection are each performed as training system-side detection by an optical training system,
- and wherein the application technology and training technology are configured as microscopy or flow cytometry.