Flexible light sheet generation by field synthesis
AssigneeBoard of Regents, The University of Texas System
InventorsReto Fiolka, Mark Kittisopikul, Bo-Jui Chang
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Abstract
A light sheet microscopy apparatus has a light source, a spatial light filter, a scanning device positioned between the light source and the spatial light filter, an imaging device, a computer readable medium, and a computer processor coupled to the light source, the scanning device, the imaging device, and the computer-readable medium. The computer processor is configured to operate the light source to generate a light beam, control the scanning device to scan the light beam across the spatial light filter to generate the light sheet, and utilize the imaging device to capture one or more images of light emitted by a sample illuminated by the one or more light sheets.
Claims23
A light sheet microscopy apparatus, comprising:
- one or more light sources;
- one or more spatial light filters;
- one or more scanning devices positioned between the one or more light sources and the one or more spatial light filters;
- one or more imaging devices;
- one or more computer readable media;
- and
- one or more computer processors coupled to the one or more light sources, the one or more scanning devices, the one or more imaging devices, and the one or more computer-readable media, wherein the one or more computer processors is configured to:
- operate the one or more light sources to generate one or more light beams;
- control the one or more scanning devices to scan the one or more light beams across the one or more spatial light filters to generate one or more light sheets;
- and
- utilize the one or more imaging devices to capture one or more images of light emitted by a sample illuminated by the one or more light sheets.
A method of operation for a light sheet microscopy apparatus, comprising:
- operating one or more light sources to generate one or more light beams;
- controlling the one or more scanning devices to scan the one or more light beams across one or more spatial light filters to generate one or more light sheets;
- and
- utilizing the one or more imaging devices to capture one or more images of light emitted by a sample illuminated by the one or more light sheets.
A light sheet microscopy apparatus, comprising:
- means for generating one or more light beams;
- means for scanning the one or more light beams across one or more spatial light filters to generate one or more light sheets;
- and
- means for capturing one or more images of light emitted by a sample illuminated by the one or more light sheets.
A computer-readable medium having instructions recorded thereon that, when enacted by one or more computer processors, cause the one or more computer processors to:
- operate one or more light sources to generate one or more light beams;
- control one or more scanning devices to scan the one or more light beams across one or more spatial light filters to generate one or more light sheets;
- and
- utilize one or more imaging devices to capture one or more images of light emitted by a sample illuminated by the one or more light sheets.
Dependencies are inferred from the claim text.