Centripetal microfluidic platform for lal reactive substances testing

A method for testing at least one fluid sample for LAL-reactive substances, said method comprising:

• inserting a microfluidic disc into a optical reader wherein:
• said microfluidic disc comprises at least two testing areas wherein each testing area includes:
• a reservoir portion for receiving at least one fluid sample, said reservoir portion comprising a reservoir and a reservoir outlet;
• and a distribution network portion in fluid communication with said reservoir portion;
• wherein each distribution network portion comprises a distribution network of at least four (4) testing channels, wherein each channel has a metering portion and at least one analysis chamber portion comprising an optical chamber, said metering portion being sized to meter an aliquot of said fluid sample for analysis in said optical chamber;
• and
• said reader comprises an enclosure, an optical bench, a centripetal disc drive, an inlet for introducing said fluid sample into said disc, and a controller;
• inserting said fluid sample into said inlet of said reader;
• spinning said disc until reaction velocity is reached;
• analyzing said aliquot in said optical chamber using said optical bench to obtain measurement data and/or reaction data;
• calculating testing results using calibration curves and said measurement data and/or reaction data;
• and
• reporting and/or storing said testing results.