Analyte detection method
AssigneeSysmex Corporation
InventorsKenji AKAMA, Hiroyuki NOJI
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Abstract
Disclosed is an analyte detection method, including detecting an analyte in such a state that a complex composed of the analyte in a sample of interest, a particle capable of bonding to the analyte and a trapping substance capable of bonding to the analyte is formed on a substrate, wherein the analyte is detected on the basis of an index associated with a behavior of the particle.
Claims20
An analyte detection method, comprising detecting an analyte in such a state that a complex comprising the analyte in a sample of interest, a particle capable of bonding to the analyte and a trapping substance capable of bonding to the analyte is formed on a substrate, wherein the analyte is detected on the basis of an index associated with a behavior of the particle.
An analyte detection method, comprising steps of:
- forming on a substrate a complex comprising an analyte in a sample of interest, particle capable of bonding to the analyte and a trapping substance capable of bonding to the analyte, and
- detecting the analyte on the basis of at least one index selected from the group consisting of a mean square displacement, a diffusion coefficient (D) and an average velocity (V) of the Brownian motion of the particle.
An analyte detection method, comprising steps of:
- forming on a substrate a complex comprising an analyte in a sample of interest, particle capable of bonding to the analyte and a trapping substance capable of bonding to the analyte,
- taking a still image of the behavior of the particle or a moving image of the behavior of the particle,
- calculating using the still image or the moving image at least one index selected from the group consisting of a mean square displacement, a diffusion coefficient (D) and an average velocity (V) of the Brownian motion of the particle, and
- detecting the analyte by determining that a particle of which the index is equal to or less than the threshold value of the upper limit of the index or a particle of which the index is equal to or less than the threshold value of the upper limit of the index and is equal to or more than the threshold value of the lower limit of the index is a particle to which the analyte is bonded.
Dependencies are inferred from the claim text.