Nanoparticle-enhanced enzymatic immune biosensor assay for antigen detection
AssigneeUniversity of South Florida
InventorsShyam Mohapatra, Subhra Mohapatra
Life of this patent
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Abstract
Provided are methods of detecting antigens in a sample by methods using a nanoparticle-enhanced enzymatic immune bio sensor. Methods of detecting abnormal kidney function in a subject and methods of detecting cancer in a subject are provided. Also provided are kits for detecting antigens.
Claims111
A method of detecting an antigen in a subject, the method comprising:
- (a) contacting a sample from the subject with:
- (i) a first antibody conjugated to a first nanoparticle;
- (ii) a second antibody conjugated to a second nanoparticle, wherein the nanoparticle is conjugated to an enzyme, wherein the first and second antibodies bind an antigen;
- and
- (iii) a substrate capable of being converted to a product by the enzyme;
- (b) determining the level of the product;
- (c) comparing the level of product to a reference level of product;
- thereby detecting the level of antigen.
A kit for detecting an antigen in a subject, the kit comprising:
- (a) a first antibody conjugated to a first nanoparticle;
- (b) a second antibody conjugated to a second nanoparticle, wherein the nanoparticle is conjugated to an enzyme, wherein the first and second antibodies bind an antigen;
- and
- (c) a substrate capable of being converted to a product by the enzyme.
A method of detecting abnormal kidney function in a subject, the method comprising:
- (a) obtaining a sample from the subject;
- (b) contacting the sample with:
- (i) a first antibody conjugated to a first nanoparticle;
- (ii) a second antibody conjugated to a second nanoparticle, wherein the nanoparticle is conjugated to an enzyme, wherein the first and second antibodies bind an antigen;
- and
- (iii) a substrate capable of being converted to a product by the enzyme
- (c) determining the level of the product;
- (d) comparing the level of product to a reference level of product;
- and
- (e) identifying the subject as having abnormal kidney function when the level of the product is different than the level of product from a reference level of product.
A kit for detecting abnormal kidney function in a subject, the kit comprising:
- (a) a first antibody conjugated to a first nanoparticle;
- (b) a second antibody conjugated to a second nanoparticle, wherein the nanoparticle is conjugated to an enzyme, wherein the first and second antibodies bind an antigen;
- and
- (c) a substrate capable of being converted to a product by the enzyme.
A method of detecting cancer in a subject, the method comprising:
- (a) obtaining a sample from the subject;
- (b) contacting the sample with:
- (i) a first antibody conjugated to a first nanoparticle;
- (ii) a second antibody conjugated to a second nanoparticle, wherein the nanoparticle is conjugated to an enzyme, wherein the first and second antibodies bind an antigen;
- and
- (iii) a substrate capable of being converted to a product by the enzyme
- (c) determining the level of the product;
- (d) comparing the level of product to a reference level of product;
- and
- (e) identifying the subject as having cancer when the level of the product is different than the level of product from a reference level of product.
A kit for detecting cancer in a subject, the kit comprising:
- (a) a first antibody conjugated to a first nanoparticle;
- (b) a second antibody conjugated to a second nanoparticle, wherein the nanoparticle is conjugated to an enzyme, wherein the first and second antibodies bind an antigen;
- and
- (c) a substrate capable of being converted to a product by the enzyme.
The kit of claim 63, further comprising a glucose meter.
A method of detecting an antigen in a subject;
- the method comprising:
- (a) obtaining a sample from a subject;
- (b) contacting a sample from the subject with:
- (i) an antigen specific antibody conjugated to a nanoparticle;
- (ii) an antigen conjugated to an enzyme;
- and
- (iii) a substrate capable of being converted to a product by the enzyme;
- (c) determining the level of product;
- and
- (d) comparing the level of product to a reference level of product;
- thereby detecting the level of biomarker.
The method of claim 65, wherein the biomarker is a kidney function biomarker.
A kit for detecting an antigen in a sample, the kit comprising:
- (a) an antigen specific antibody conjugated to a nanoparticle;
- (b) an antigen conjugated to an enzyme;
- and
- (c) a substrate capable of being converted to a product by the enzyme.
A method of detecting abnormal kidney function in a subject, the method comprising:
- (a) obtaining a sample from the subject;
- (b) contacting the sample with:
- (i) an antigen specific antibody conjugated to a nanoparticle;
- (ii) an antigen conjugated to an enzyme;
- and
- (iii) a substrate capable of being converted to a product by the enzyme;
- (c) determining the level of product;
- (d) comparing the level of product to a reference level of product;
- and
- (e) identifying the subject as having abnormal kidney function when the level of product is different than the level of product from a reference level of product.
A kit for detecting abnormal kidney function in a subject, the kit comprising:
- (a) an antigen specific antibody conjugated to a nanoparticle;
- (b) an antigen conjugated to an enzyme;
- and
- (c) a substrate capable of being converted to a product by the enzyme.
A method of detecting cancer in a subject, the method comprising:
- (a) obtaining a sample from the subject;
- (b) contacting the sample with:
- (i) an antigen specific antibody conjugated to a nanoparticle;
- (ii) an antigen conjugated to an enzyme;
- and
- (iii) a substrate capable of being converted to a product by the enzyme;
- (c) determining the level of product;
- (d) comparing the level of product to a reference level of product;
- and
- (e) identifying the subject as having abnormal kidney function when the level of product is different than the level of product from a reference level of product.
A kit for detecting cancer in a subject, the kit comprising:
- (a) an antigen specific antibody conjugated to a nanoparticle;
- (b) an antigen conjugated to an enzyme;
- and
- (c) a substrate capable of being converted to a product by the enzyme.
Dependencies are inferred from the claim text.