Enzyme-linked assay for measurement of porphobilinogen (pbg)
AssigneeBoard of Regents, The University of Texas System
InventorKarl E. Anderson
Life of this patent
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Abstract
The present invention includes a method and kit for detecting porphobilinogen fluorometrically comprising: obtaining a sample suspected of having a porphobilinogen; catalyzing a reaction in the sample with a porphobilinogen deaminase to form a porphyrin; exposing the porphyrin to a light source having a 399 nm wavelength; detecting fluorometrically the amount of porphyrin in the sample; and correlating the amount of porphyrin in the sample to an amount of porphobilinogen in the sample, wherein the ratio of porphobilinogen to porphyrin is 4:1.
Claims18
A method for detecting porphobilinogen fluorometrically comprising:
- obtaining a sample suspected of having a porphobilinogen;
- catalyzing a reaction in the sample with a porphobilinogen deaminase to form a porphyrin reaction sample;
- exposing the porphyrin to a light source having a 399 nm wavelength;
- detecting fluorometrically the amount of porphyrin in the sample at 620 to 700 nm;
- and
- correlating the amount of porphyrin in the reaction sample to an amount of porphobilinogen in the sample, wherein the ratio of porphobilinogen to porphyrin is 4:1.
A method for treating a patient having elevated porphobilinogen comprising:
- obtaining a urine sample suspected of having a porphobilinogen;
- catalyzing a reaction of the urine sample with a porphobilinogen deaminase to form a porphyrin;
- exposing the porphyrin to a light source having a 399 nm wavelength;
- detecting fluorometrically the amount of porphyrin in the urine sample;
- correlating the amount of porphyrin in the urine sample to an amount of porphobilinogen in the sample, wherein the ratio of porphobilinogen to porphyrin is 4:1, wherein the patient is suspected of having at least one of acute intermittent porphyria (AIP), hereditary coproporphyria (HCP), or variegate porphyria (VP);
- and
- treating the patient with elevated porphobilinogen with carbohydrate loading or hemin, RNA interference, or gene therapy.
A kit comprising:
- a vial for collecting a sample;
- a vial with a porphobilinogen deaminase;
- a solution for catalyzing a porphobilinogen deaminase reaction with a porphobilinogen in the sample;
- and
- instructions for correlating the amount of porphyrin in the sample to an amount of porphobilinogen in the sample, wherein the ratio of porphobilinogen to porphyrin is4:1, after exposing the porphyrin to a 399 nm wavelength light source.
Dependencies are inferred from the claim text.