Methods for generating and screening compartmentalised peptide libraries
AssigneeUniversity of Southampton
InventorsAli TAVASSOLI, Catrin SOHRABI, Martin FISCHLECHNER
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Abstract
A method for co-compartmentalising a cyclic polypeptide with a polynucleotide encoding the cyclic polypeptide, comprising the steps of a) forming a compartment containing a polynucleotide encoding the cyclic polypeptide, b) expressing a polypeptide from the polynucleotide, and c) cyclising the polypeptide. Co-compartmentalised cyclic polypeptides and encoding polynucleotides. Libraries of co-compartmentalised cyclic polypeptide and encoding polynucleotide. Methods for screening libraries of co-compartmentalised cyclic polypeptide and encoding polynucleotide. Incorporation of non-canonical nucleic acids into such libraries.
Claims20
A method for co-compartmentalising a cyclic polypeptide with a polynucleotide encoding the cyclic polypeptide, comprising the steps of:
- a) forming a compartment containing a polynucleotide encoding the cyclic polypeptide;
- b) expressing a polypeptide from the polynucleotide;
- and
- c) cyclising the polypeptide.
A compartment comprising:
- a) a polynucleotide comprising a sequence encoding an N-terminal intein fragment, followed by a sequence encoding a cyclic polypeptide, followed by a sequence encoding a C-terminal intein fragment;
- and
- b) the cyclic peptide.
A kit comprising:
- a) a microfluidics device;
- b) a polynucleotide encoding an N-terminal intein fragment;
- and
- c) a polynucleotide encoding a C-terminal intein fragment.
Dependencies are inferred from the claim text.