Nir spectroscopy method for fatty acid content of oilseeds

A NIR method for fatty acid content of oilseeds, comprising the steps of:

• S1:
• providing oilseed samples, and acquiring NIR spectra of the oilseed samples by using a near infrared spectrometer;
• S2:
• performing standard normal transformation and second derivative data preprocessing on the acquired NIR spectra of the oilseed samples to obtain preprocessed NIR spectra, and establishing the NIR spectrum database of oilseeds;
• S3:
• establishing a fatty acid database of oilseeds based on gas chromatography to obtain the relative content of fatty acids in each oilseed sample;
• using a method for measuring oil content of oilseeds to obtain information on the oil content of each oilseed sample;
• converting the relative content of fatty acids into absolute content;
• establishing the oilseed fatty acid database composed of the relative content, absolute content and correction coefficient of fatty acids as effective information;
• S4:
• establishing a prediction model of fatty acids in oilseeds by dividing the oilseed samples into training set and validation set;
• using a variable selection method in combination with NIR spectra in the training set corresponding to different indicators in the fatty acid database of oilseeds to select the corresponding important wavelengths, establishing the prediction model of fatty acids in oilseeds by using the selected important wavelengths through a chemometric method combined with the absolute content and the correction coefficient of fatty acids;
• and validating the prediction model of fatty acids by the validation set;
• and
• S5:
• providing a sample for testing;
• acquiring the NIR spectrum of the tested sample by the infrared spectrometer;
• preprocessing by the preprocessing method in step S2;
• and importing the preprocessed NIR spectrum into the prediction model of fatty acids established in step S4 to obtain the predicted fatty acid content of the tested sample.