In vitro method for determining the adsorbing capacity of an insoluble adsorbant
AssigneeAlbireo AB
InventorsPer-Göran Gillberg, Anna-Maria Tivert, Mike Frodsham, Jamie Farrar
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Abstract
The invention relates to an in vitro method for determining the adsorbing capacity of an adsorbent having limited solubility, such as a bile acid sequestrant, under conditions simulating the mammalian gastrointestinal tract. The method is particularly useful for studying the release profiles of controlled release formulations comprising adsorbents having limited solubility.
Claims26
An in vitro method for determining the adsorbing capacity of an adsorbent having limited solubility, under conditions simulating the mammalian gastrointestinal tract, wherein the method comprises the steps of:
- a) providing a liquid medium representative of at least one region of the gastrointestinal tract;
- b) adding to said liquid medium defined amounts of an adsorbent and a marker compound;
- and
- c) incubating the mixture obtained in step (b) and measuring the amount of free marker compound in said mixture.
An in vitro method for determining the adsorbing capacity of an adsorbent having limited solubility, under conditions simulating the mammalian gastrointestinal tract, wherein the method comprises the steps of:
- a) providing a liquid medium representative of the stomach;
- b) adding to said liquid medium a defined amount of an adsorbent;
- c) incubating the mixture obtained in step (b);
- d) converting the mixture of step c) to a medium representative of the small intestine;
- e) adding a defined amount of a marker compound to the mixture of step d);
- f) incubating the mixture obtained in step (e) and measuring the amount of free marker compound in said mixture;
- g) converting the mixture of step f) to a medium representative of the colon;
- and
- h) incubating the mixture obtained in step (g) and measuring the amount of free marker compound in said mixture.
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Dependencies are inferred from the claim text.