Compositions and methods for analyzing heterogeneous samples
AssigneeLineage Biosciences, Inc.
InventorsDan Seligson, Thomas Snyder
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Abstract
Methods and compositions for detecting molecules in a heterogeneous sample are disclosed. The methods and compositions disclosed herein may be used for the treatment of a disease or condition characterized by the presence of nucleic acids from at least two different genomic sources. Additionally, the methods and compositions disclosed herein may be used to diagnose, predict, or monitor the status or outcome of a disease or condition characterized by the presence of nucleic acids from at least two different genomic sources. The heterogeneous samples may be from a transplant recipient, a chimeric individual, a subject suffering from a pathogenic infection, or a subject suffering from a different condition such as cancer.
Claims21
-74. (canceled)
A method comprising the steps of:
- a. detecting via a nucleic acid sequencing reaction, a quantity of circulating cell-free nucleic acids in a biological sample comprising circulating cell-free nucleic acids obtained from a subject with cancer who has been previously administered a therapeutic regimen,
- wherein the circulating cell-free nucleic acids comprise nucleic acids from cancer tissue and normal tissue and are mRNA or DNA, and the circulating cell-free nucleic acids from the cancer tissue comprise a nucleic acid region having a genetic variation, and
- b. modifying the therapeutic regimen to be administered to the subject based on the quantity of the circulating cell-free nucleic acids from the cancer tissue in the biological sample, wherein the therapeutic regimen is increased if the quantity of the circulating cell-free nucleic acids from the cancer tissue is greater than 0.5% of the total circulating cell-free nucleic acids in the biological sample.
Dependencies are inferred from the claim text.