Methods of detecting cell-free dna in biological samples
AssigneeCornell University
InventorsIwijn DE VLAMINCK, John Richard LEE, Philip Smith BURNHAM, Alexandre Pellan CHENG, Manikkam SUTHANTHRIAN, Darshana DADHANIA
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Abstract
The present disclosure is directed to methods of detecting cell-free DNA (cfDNA) in biological samples and using it to quantify organ damage and identify pathogens. In some aspects, the biological samples are from patients who have undergone solid-organ transplantation. The disclosure is also directed to methods of detecting and analyzing methylation patterns in cell-free DNA from organ transplant patients to identify the presence of pathogens as well as quantify contributing tissue proportions as a measurement of the host response.
Claims21
A method comprising:
- (a) obtaining a sample of a subject at risk of a urinary tract or peritoneal infection, wherein the sample is selected from the group consisting of a urine sample and a peritoneal dialysis fluid sample;
- (b) preparing a sequencing library from the sample, wherein the sequencing library comprises genomic DNAs present in the sample;
- (c) sequencing the DNAs in the sequencing library;
- and
- (d) detecting a non-host DNA in the library.
Dependencies are inferred from the claim text.