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AssigneesBoe Technology Group Co., Ltd., Beijing Boe Optoelectronics Technology Co., Ltd.
InventorsPeizhi CAI, Fengchun PANG, Yue GENG, Le GU, Chuncheng CHE
Estimating the term…
A gene sequencing substrate and a method for manufacturing the same, and a gene sequencing device are provided. It belongs to the technical field of gene sequencing, and can solve the problem of high cost of the high-throughput sequencing chip in the prior art. The gene sequencing substrate of the present disclosure comprises a plastic material with concave structures as base substrate, and the concave structures serve as reaction cells. Since the base substrate has plasticity, the concave structures can be formed by a simple process to reduce the cost of the gene sequencing substrate. Meanwhile, a first protective layer may be provided on the inner wall of the concave structures for preventing the inner wall of the concave structures from being corroded by the reaction liquid.
A gene sequencing substrate comprising a plastic base substrate with a surface having concave structures.
Dependencies are inferred from the claim text.
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By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…
Involving nucleic acids: methods for sequencing
Microtiter plates: microwell devices, i.e. having large numbers of wells
Specific forms of parts of containers: bottom walls
Term end not estimated
The present application claims priority to the Chinese Patent Application No. 201810004294.3 entitled “gene sequencing substrate and method for manufacturing the same, gene sequencing device” filed on Jan. 3, 2018, the entire content of which is incorporated herein by reference.
The present disclosure belongs to the technical field of gene sequencing, and particularly relates to a gene sequencing substrate and a method for manufacturing the same, a gene sequencing device and a gene sequencing method.
Gene sequencing technology is the most commonly used technology in modern molecular biology research. The current predominant sequencing technology in the market is high-throughput sequencing technology.
FIG. 1 is a schematic view showing a process of manufacturing a gene sequencing substrate in the prior art;
FIG. 2 is a schematic cross-sectional view of the gene sequencing substrate according to an embodiment of the present disclosure;
FIG. 3 is a schematic cross-sectional view of the gene sequencing substrate according to an embodiment of the present disclosure;
For a better understanding of the embodiments of the present disclosure, the present disclosure will be further described in detail below with reference to the accompanying drawings and specific embodiments.
Some embodiments of the present disclosure provide a gene sequencing substrate comprising a plastic base substrate which has a surface with concave structures thereon. The concave structures can be used as reaction cells for gene sequencing. The gene sequencing substrate may further comprise a first protective layer disposed on the base substrate which at least covers the concave structures, and the first protective layer has enough chemical resistance to a reagent for the gene sequencing and adhesivity with the plastic base substrate, so that results of the gene sequencing can be assured and the first protective layer would not shed off after the gene sequencing.
In one embodiment, as shown in FIG. 2, the gene sequencing substrate comprises a plastic base substrate 51 having a surface with concave structures 52 thereon. The concave structures 52 serve as reaction cells for gene sequencing. The term of “plastic base substrate” herein means that, when being subjected to an external force, the base substrate is significantly deformed but not destroyed. Since the base substrate 51 has plasticity, the concave structures 52 can be formed by a simple process, thereby ensuring the production efficiency of the gene sequencing substrate and reducing manufacturing costs. Further, a first protective layer 53 is provided on the base substrate to protect the surface of the concave structures 52 from being corroded by the reaction liquid.