Kits for single-step analyte detection with process control
AssigneeGen-Probe Incorporated
InventorsSunghae A. JOO, Janel M. DOCKTER
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Abstract
Kits for detecting analyte polynucleotides and an internal control in a sample. Included in the kit are an internal control polynucleotide and amplification reagents to co-amplify a first analyte polynucleotide and the internal control. Also included are first and second hybridization probes, each having a label indistinguishable from the other. The probes are respectively capable of hybridizing with a first analyte amplicon and an internal control amplicon. The first and second labels are indistinguishable homogeneous labels.
Claims20
A kit for determining the presence or absence of analyte polynucleotides in a sample, the kit comprising:
- an internal control polynucleotide;
- one or more amplification reagents sufficient to co-amplify a first analyte polynucleotide and the internal control polynucleotide in a nucleic acid amplification reaction to produce an internal control amplicon, and, if the sample contains the first analyte polynucleotide, a first analyte amplicon;
- and
- a first hybridization probe having a first label, the first hybridization probe being capable of forming a first detectable hybrid with the first analyte amplicon, but incapable of forming a detectable hybrid with the internal control amplicon, and
- a second hybridization probe having a second label, the second hybridization probe being capable of forming a second detectable hybrid with the internal control amplicon, but incapable of forming a detectable hybrid with the first analyte amplicon,
- wherein the first and second labels are indistinguishable from each other, and
- wherein the first and second labels comprise indistinguishable homogeneous labels.
Dependencies are inferred from the claim text.