Compositions, kits and related methods for the detection and/or monitoring of listeria
AssigneeGen-Probe Incorporated
InventorsMichael R. Reshatoff, Kristin W. Livezey, James J. Hogan
Life of this patent
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Abstract
Provided are compositions, kits, and methods for the identification of Listeria. In certain aspects and embodiments, the compositions, kits, and methods may provide improvements in relation to specificity, sensitivity, and speed of detection.
Claims20
A set of oligonucleotides configured to amplify in an amplification reaction a Listeria nucleic acid sequence from L. monocytogenes, L. innocua, L. grayi, L. ivanovii, L. welshimeri, L. murrayi, and L. seeligeri without substantial amplification of a Brochothrix thermosphacta nucleic acid or an Erysipelothrix rhusiopathiae nucleic acid comprising a first T7 provider oligonucleotide and a first primer oligonucleotide,
- wherein the first T7 provider oligonucleotide targets a sequence in a first Listeria nucleic acid region corresponding to nucleotide positions of about 15-91 of SEQ ID NO:
- 103;
- and
- wherein the first primer oligonucleotide targets a sequence in a second Listeria nucleic acid region corresponding to nucleotide positions of about 90-156 of SEQ ID NO:
- 103.
A set of oligonucleotides for detecting a Listeria nucleic acid sequence in a sample comprising a T7 provider oligonucleotide, a primer oligonucleotide, and a detection oligonucleotide, wherein the T7 provider oligonucleotide and the primer oligonucleotide are configured amplify in an amplification reaction a nucleic acid sequence from L. monocytogenes, L. innocua, L. grayi, L. ivanovii, L. welshimeri, L. murrayi, and L. seeligeri without substantial amplification of a Brochothrix thermosphacta nucleic acid or an Erysipelothrix rhusiopathiae nucleic acid, wherein the first T7 provider oligonucleotide targets a sequence in a first Listeria nucleic acid region corresponding to nucleotide positions of about 15-91 of SEQ ID NO:
- 103, and wherein the first primer oligonucleotide targets a sequence in a second Listeria nucleic acid region corresponding to nucleotide positions of about 90-156 of SEQ ID NO:
- 103.
Dependencies are inferred from the claim text.