Production method for substance using atp

A method of producing a substance, comprising synthesizing a molecule at least by mixing substrates, a synthase, adenosine triphosphate (ATP), a polyphosphate kinase 2, and a polyphosphoric acid mixture,

• wherein the polyphosphoric acid mixture comprises 50% or more of polyphosphoric acid with a degree of polymerization of not less than 15,
• wherein adenosine diphosphate (ADP) is generated from the ATP during the synthesis,
• wherein the synthesis is coupled with an ATP regeneration reaction in which the ATP is regenerated by the polyphosphate kinase 2 from the ADP and the polyphosphoric acid, and
• wherein the polyphosphate kinase 2 is polyphosphate kinase 2 having a sequence identity of not less than 80% to at least one selected from the group consisting of:
• polyphosphate kinase 2 derived from Pseudomonas aeruginosa;
• polyphosphate kinase 2 derived from Synechococcus sp. PCC6312;
• polyphosphate kinase 2 derived from Corynebacterium efficiens;
• polyphosphate kinase 2 derived from Kineococcus radiotolerans;
• polyphosphate kinase 2 derived from Pannonibacter indicus;
• polyphosphate kinase 2 derived from Deinococcus radiodurans K1;
• polyphosphate kinase 2 derived from Gulbenkiania indica;
• polyphosphate kinase 2 derived from Arthrobactor aurescens TC1;
• polyphosphate kinase 2 derived from Thiobacillus denitrificans ATCC25259;
• and polyphosphate kinase 2 derived from Pseudomonas fluorescens.