US 2020/0048670 A1 · Method for Improving Yield and Production Intensity of Gluconobacter oxydans Sorbose

Abstract

The present disclosure relates to a method for improving the yield and production intensity of Gluconobacter oxydans (G. oxydans) sorbose, and belongs to the technical field of fermentation engineering. By knocking out genes related to formation of D-sorbitol or L-sorbose metabolic by-products in G. oxydans, the formation of the by-products is reduced, and the efficiency of transforming D-sorbitol into L-sorbose is improved, thereby improving the yield and production intensity of L-sorbose. A recombinant strain G. oxydan-11 constructed by the present disclosure, compared with a control strain, has an L-sorbose transformation rate of 96.12%, which is 4.47% higher than that of a wild strain, has a production intensity of 14 g/L·h, which is 14.7% higher than that of the wild strain, and has a fructose by-product content of only 5.6 g/L, which is 45.6% lower than that of the wild strain.

Claims15

Dependent claims

A method for improving yield and production intensity of L-sorbose, comprising knocking out genes related to formation of an L-sorbose metabolic by-product, wherein the genes related to formation of the L-sorbose metabolic byproduct comprise at least one of GDH, GA-5-DH, XDH2, ALDH, XDH, sDH SLC, PTS, PQQ-dependent DH3, NADH-DH, and NADH-dependent ADH genes;

and
the GDH contains a nucleotide sequence set forth in SEQ ID NO. 1;
the GA-5-DH contains a nucleotide sequence set forth in SEQ ID NO. 2;
the XDH2 contains a nucleotide sequence set forth in SEQ ID NO. 3;
the ALDH contains a nucleotide sequence set forth in SEQ ID NO. 4;
the XDH contains a nucleotide sequence set forth in SEQ ID NO. 5;
the sDH SLC contains a nucleotide sequence set forth in SEQ ID NO. 6;
the PTS contains a nucleotide sequence set forth in SEQ ID NO. 7;
the PQQ-dependent DH3 contains a nucleotide sequence set forth in SEQ ID NO. 8;
the NADH-DH contains a nucleotide sequence set forth in SEQ ID NO. 9;
and the NADH-dependent ADH contains a nucleotide sequence set forth in SEQ ID NO. 10.

8 more elements

A genetically engineered strain for producing L-sorbose, wherein genes related to formation of major metabolic byproducts generated by synthesis of sorbose in a metabolic pathway of L-sorbose produced are knocked out;

the major metabolic byproducts comprise fructose;
and the genes related to formation of the major metabolic byproducts comprise at least one of GDH, GA-5-DH, XDH2, ALDH, XDH, sDH SLC, PTS, PQQ-dependent DH3, NADH-DH, and NADH-dependent ADH genes.

Dependencies are inferred from the claim text.

Method for Improving Yield and Production Intensity of Gluconobacter oxydans Sorbose

Life of this patent

Method for Improving Yield and Production Intensity of Gluconobacter oxydans Sorbose

Life of this patent

Abstract

Claims15