Increasing the Content of Long Chain Fatty Acids in Seed Oil
AssigneesDonald Danforth Plant Science Center, The Curators of the University of Missouri
InventorsXuemin Wang, Maoyin Li
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Abstract
Provided are plants that express, or overexpress, a pPLAIIIδ protein. Constitutive or seed-specific expression of pPLAIIIδ protein in Arabidopsis increases seed oil content, the amount of C20 and C22 fatty acids, and the amount of C56, C58, and C60 triacylglycerols, effectively resulting in significantly higher oil yield per plant. Use of pPLAIIIδ is therefore an effective biotechnological tool to significantly increase plant yield, including oil, and the amount of high value long chain fatty acids in agricultural and horticultural crops, especially oilseed crops.
Claims21
A transgenic oilseed plant, other than Arabidopsis, the genome of which comprises a heterologous nucleotide sequence that encodes a pPLAIIIδ protein, which heterologous nucleotide sequence is expressed or overexpressed.
A method selected from the group consisting of:
- (i) producing an enhanced amount of oil in seeds of an oilseed plant compared to the amount of oil produced in seeds by an otherwise identical control oilseed plant grown under the same conditions;
- (ii) producing oil in seeds of an oilseed plant containing an enhanced amount of C20 and C22 fatty acids compared to the amount of C20 and C22 fatty acids in oil produced in seeds by an otherwise identical control oilseed plant grown under the same conditions;
- (iii) producing oil in seeds of an oilseed plant containing an enhanced amount of C56, C58, and C60 triacylglycerols compared to the amount of C56, C58, and C60 triacylglycerols in oil produced in seeds by an otherwise identical control oilseed plant grown under the same conditions;
- (iv) producing an enhanced amount of oil in seeds of an oilseed plant containing an enhanced amount of C20 and C22 fatty acids compared to the amount of oil and C20 and C22 fatty acids produced in seeds by an otherwise identical control oilseed plant grown under the same conditions;
- (v) producing an enhanced amount of oil in seeds of an oilseed plant containing an enhanced amount of C56, C58, and C60 triacylglycerols compared to the amount of oil and C56, C58, and C60 triacylglycerols produced in seeds by an otherwise identical control oilseed plant grown under the same conditions;
- (vi) producing an enhanced amount of oil in seeds of an oilseed plant containing an enhanced amount of C20 and C22 fatty acids and an enhanced amount of C56, C58, and C60 triacylglycerols compared to the amount of oil, C20 and C22 fatty acids, and C56, C58, and C60 triacylglycerols, respectively, produced in seeds by an otherwise identical control oilseed plant grown under the same conditions;
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- (vii) producing enhanced levels of mRNA transcripts for genes in the Kennedy pathway in seeds of an oilseed plant compared to the levels of mRNA transcripts for genes in the Kennedy pathway produced in seeds by an otherwise identical control oilseed plant grown under the same conditions,
- wherein said oilseed plant is a plant other than Arabidopsis,
- wherein said method comprises expressing or overexpressing a protein having at least 95% sequence similarity to Arabidopsis pPLAIIIδ protein comprising the amino acid sequence encoded by Arabidopsis Genome Initiative Database Accession At3g63200 in said oilseed plant, and
- wherein said pPLAIIIδ protein exhibits enzymatic activity in the range of from about 75% to about 125% or more of the enzymatic activity of said Arabidopsis pPLAIIIδ protein.
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Dependencies are inferred from the claim text.