Gene editing and transgene free mutant plants

A recombinant engineered, non-naturally occurring gene editing system comprising one or more vectors comprising:

• (a) at least one first regulatory element operable in a plant cell and operably linked to at least one nucleotide sequence encoding a CRISPR-Cas system guide RNA (gRNA) for targeting a target sequence in a plant,
• (b) a second regulatory element operable in a plant cell operably linked to a nucleotide sequence encoding a class 2 CRISPR-associated nuclease, and
• (c) a third regulatory element operable in a plant cell operably linked to a fluorescent reporter and
• optionally (d) a fourth regulatory element linked to an antibiotic resistance gene,
• wherein component (a) is located on the same or different vector than components (b) and (c), whereby the guide RNA targets the target sequence and the CRISPR-associated nuclease cleaves the DNA molecule, whereby expression of the at least one gene product is altered;
• and, wherein the CRISPR-associated nuclease and the guide RNA do not naturally occur together.