Yeast strains with selected or altered mitotypes and methods of making and using the same
AssigneeWisconsin Alumni Research Foundation
InventorsChris Todd Hittinger, David Peris Navarro, EmilyClare Patricia Baker
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Abstract
Herein we demonstrate that the mitochondrial genome influences temperature tolerance in Saccharomyces yeasts. The present invention provides methods for manipulating the mitotype of yeast, including methods to produce synthetic yeast hybrids with a selected mitotype and methods to exchange the native mitochondrial DNA (mtDNA) present in polyploid yeast with mtDNA from a desired source. Saccharomyces cerevisiae x Saccharomyces eubayanus hybrids with selected mitotypes are also provided. The yeast and methods of the present invention may be utilized in a variety of applications, including in fermentation to produce beer and wine.
Claims33
A method of making a hybrid yeast strain having a selected mitotype comprising:
- treating a first yeast strain with a mitochondria elimination agent to produce a first mitochondria-null yeast strain, and
- mating the first mitochondria-null yeast strain with a second yeast strain comprising mitochondria to produce the yeast strain having the selected mitotype.
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A method of making a yeast strain with an altered mitotype comprising:
- treating a first yeast strain with a mitochondria elimination agent to produce a first mitochondria-null yeast strain, and
- mating the first mitochondria-null yeast strain with a second karyogamy-deficient yeast strain comprising mitochondria DNA (mtDNA) to produce the yeast strain having the altered mitotype, wherein the first yeast strain is polyploid.
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A Saccharomyces cerevisiae x Saccharomyces eubayanus hybrid comprising mitochondria DNA (mtDNA), wherein at least 90% of the mtDNA is from Saccharomyces cerevisiae.
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A Saccharomyces cerevisiae x Saccharomyces eubayanus hybrid comprising mitochondrial DNA (mtDNA), wherein at least 90% of the mtDNA is from Saccharomyces eubayanus.
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Dependencies are inferred from the claim text.