Method for preparing heterodimer snake venom protein
AssigneeZhaoke Pharmaceutical (HEFEI) Company Limited
InventorsXiangrong Dai, Xiaoyi Li, Fang Qian
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Abstract
Provided are recombinant plasmids containing the heterodimeric snake venom protein Agkisacutacin A chain gene and Agkisacutacin B chain gene, respectively, cell strains containing the recombinant plasmids, and a method for expressing the heterodimeric snake venom protein Agkisacutacin. The expression level of Agkisacutacin in the present method exceeds 10 mg/L, and the purity level can reach more than 95% by means of two steps of purification.
Claims11
Plasmid pPIC9K-A shown in FIG. 1, wherein the sequence of Agkisacutacin A chain gene is shown in SEQ ID NO. 1.
Plasmid pUCZ-B shown in FIG. 2, wherein the sequence of Agkisacutacin B chain gene is shown in SEQ ID NO.2.
(canceled)
Dependencies are inferred from the claim text.