Integration of mesa receptors and promotors to implement customized cellular function

A system comprising:

• (i) first and second exogenous extracellular sensors, and third and fourth exogenous extracellular sensors;
• and (ii) a recombinant cell comprising an endogenous target gene operably linked to a recombinant hybrid promoter sequence comprising a minimal promoter for inducing transcription and the hybrid promoter sequence further comprising interspaced transcription regulator binding sites upstream of the minimal promoter that bind two or more transcription regulators of the extracellular sensors;
• the first and third exogenous extracellular sensors comprising:
• a) a ligand binding domain, b) a transmembrane domain, c) a protease cleavage site, and d) a functional domain comprising a transcription factor, wherein the transcription regulator of the first exogenous extracellular sensor is different than the transcription factor of the third exogenous extracellular sensor;
• the second and fourth exogenous extracellular sensors comprising:
• e) a ligand binding domain, f) a transmembrane domain, and g) a protease domain;
• wherein:
• the ligand binding domain of the first exogenous sensor and the ligand binding domain of the second exogenous sensor bind to the same ligand to form a tertiary complex;
• the ligand binding domain of the third exogenous sensor and the ligand binding domain of the fourth exogenous sensor bind to the same ligand to form a tertiary complex;
• the ligand binding domains of the first and second exogenous sensors bind to a different ligand than the ligand that is bound by the ligand binding domains of the third and fourth exogenous sensors;
• and
• the transcription regulator binding sites upstream of the minimal promoter of the expression vector comprise binding sites for the transcription regulator of the first extracellular sensor and binding sites for the transcription regulator of the third extracellular sensor.