Modified glucoamylase enzymes and yeast strains having enhanced bioproduct production
AssigneeCargill, Incorporated
InventorsChristopher K. MILLER, Ana NEGRETE-RAYMOND, Jon VELDHOUSE, Amit VAS
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Abstract
The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having a S. cerevisiae mating factor alpha 2 (Sc MFα2) or repressible acid phosphatase (Sc PHO5) secretion signal.
Claims12
1-43. (canceled)
A host cell comprising a heterologous nucleic acid encoding for a polypeptide comprising (a) a secretion signal amino acid sequence having 90% or greater identity to SEQ ID NO:10 or SEQ ID NO:11 and (b) a glucoamylase amino acid sequence from a yeast, fungal, or bacterial glucoamylase polypeptide, wherein the polypeptide has glucoamylase activity.
The host cell of claim 45, wherein the glucoamylase amino acid sequence has 90% or greater sequence identity to amino acids 19-515 of SEQ ID NO:12.
Dependencies are inferred from the claim text.