Nucleic Acid Molecules Comprising a Variant RpoC Coding Sequence
AssigneeCj Cheiljedang Corporation
InventorsJi Sun LEE, Dong Eun CHANG, So Young KIM
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Abstract
A nucleic acid molecule comprising a variant rpoC coding sequence is disclosed. The variant rpoC coding sequence encodes a variant RpoC which regulates copy number of a plasmid. Also disclosed are a recombinant microorganism comprising the nucleic acid molecule, a method for regulating copy number of a subject vector in the recombinant microorganism, and a method for making a target product by use of the recombinant microorganism.
Claims24
A nucleic acid molecule comprising a variant rpoC RNA-polymerase β′ subunit protein coding sequence (variant rpoC coding sequence), wherein:
- the variant rpoC coding sequence encodes a variant RpoC RNA-polymerase β′ subunit protein (variant RpoC);
- and
- the variant RpoC comprises an R47C substitution, with numbering of the R47C substitution defined based on wild-type RpoC RNA-polymerase β′ subunit protein (wild-type RpoC) of Escherichia coli.
A gene replacement vector comprising a variant rpoC coding sequence and a gene replacement sequence, wherein the variant rpoC coding sequence encodes a variant RpoC N-terminal domain comprising SEQ ID NO:
- 28;
- and
- the gene replacement sequence for replacing an endogenous rpoC coding sequence in a chromosome of a microorganism with the variant rpoC coding sequence.
Dependencies are inferred from the claim text.