Cell

A kit which comprises a first nucleic acid sequence encoding a first chimeric antigen receptor (CAR) and a second nucleic acid sequence encoding a second CAR,

• wherein the first CAR comprises a phosphorylation amplifying endodomain and wherein the second CAR comprises an activating endodomain
• and wherein:
• (i) the first nucleic acid sequence has the following structure:
• AgB1-spacer1-TM1-Pa
• in which:
• AgB1 is a nucleic acid sequence encoding an antigen-binding domain of the first CAR;
• spacer 1 is a nucleic acid sequence encoding a spacer of the first CAR;
• TM1 is a nucleic acid sequence encoding a transmembrane domain of the first CAR;
• and
• Pa is a nucleic acid sequence encoding the phosphorylation amplifying endodomain of the first CAR;
• and
• (ii) the second nucleic acid sequence has the following structure:
• AgB2-spacer2-TM2-endo
• in which:
• AgB2 is a nucleic acid sequence encoding an antigen-binding domain of the second CAR;
• spacer 2 is a nucleic acid sequence encoding a spacer of the second CAR;
• TM2 is a nucleic acid sequence encoding a transmembrane domain of the second CAR;
• and
• endo is a nucleic acid sequence encoding the activating endodomain of the second CAR.