Methods and products for transfection

A method for reprogramming a differentiated cell to a less differentiated state, comprising:

• (a) providing a differentiated cell;
• (b) culturing the differentiated cell;
• and
• (c) transfecting the differentiated cell with one or more synthetic RNA molecules,
• wherein the one or more synthetic RNA molecules include at least one RNA molecule encoding one or more reprogramming factors selected from the group consisting of Oct4 protein, Sox2 protein, Klf4 protein, c-Myc protein, I-Myc protein, Tert protein, Nanog protein, and Lin28 protein;
• wherein the transfecting results in the cell expressing the one or more reprogramming factors to result in the cell being reprogrammed to a less differentiated state;
• and
• wherein step (c) occurs in the presence of a medium containing ingredients that support reprogramming of the differentiated cell to a less differentiated state.

A method for reprogramming a non-pluripotent cell, comprising:

• (a) providing a non-pluripotent cell;
• (b) culturing the non-pluripotent cell;
• and
• (c) transfecting the non-pluripotent cell with one or more synthetic RNA molecules,
• wherein the one or more synthetic RNA molecules include at least one RNA molecule encoding one or more reprogramming factors selected from the group consisting of Oct4 protein, Sox2 protein, Klf4 protein, c-Myc protein, I-Myc protein, Tert protein, Nanog protein, and Lin28 protein;
• wherein the transfecting results in the cell expressing the one or more reprogramming factors to result in the cell being reprogrammed;
• and
• wherein step (c) occurs in the presence of a medium containing ingredients that support reprogramming of the differentiated cell to a less differentiated state.