Loading patent…
AssigneesInserm (Institute National De La Sante Et De La Recherche Medicale), Universite Paris-Sud
InventorsKarl BALABANIAN, Christelle FREITAS, Vincent RONDEAU
Estimating the term…
The present invention relates to a method for preparing lymphoid progenitors. The inventors took advantage of their original and relevant Warts, Hypogammaglobulinemia, Infections and Myelokathexis (WHIM) Syndrome (WS) model and the access to blood samples from five WS patients to investigate the impact of CXCR4 desensitization on BM and extra-medullary (i.e. splenic) hematopoiesis and hematopoietic stem and progenitor cells (HSPCs) recirculation. They developed, for the first time, an original in vitro system permitting to selectively expand HSPCs to obtain lymphoid progenitors by using an original cocktail of cytokines. In particular, the present invention relates to an in vitro method for preparing lymphoid progenitors by culturing HSPCs in an appropriate culture medium comprising an effective amount of a cocktail of cytokines consisting in SCF, IL-3, IL-6, IL-7, Flt-3, and CXCL12.
An in vitro method for preparing lymphoid progenitor cells, said method comprising the step of culturing hematopoietic stem and progenitor cells (HSPCs) in an appropriate culture medium comprising an effective amount of a cocktail of cytokines selected from the group consisting of SCF, IL-3, IL-6, IL-7 and Flt-3.
Dependencies are inferred from the claim text.