Modified immunoglobulin hinge regions to reduce hemagglutination
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsJie Liu, Irving L. Weissman, Ravindra Majeti
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Abstract
Polypeptide compositions comprising a human immunoglobulin constant region with a modified IgG4 hinge region having an N-terminal deletion of 1, 2, 3, 4, 5 amino acids, relative to a native human IgG4 hinge sequence are provided. In some embodiments the hinge sequence further comprises the amino acid substitution S228P (Eu numbering). Polypeptides comprising the modified sequence optionally comprise a binding moiety specific for an epitope that is present on red blood cells; and can provide for reduced agglutination of hematopoietic cells compared to a comparable polypeptide with a native hinge region.
Claims21
An immunoglobulin constant region polypeptide comprising a modified IgG4 hinge region with a deletion of from 1-5 amino acids in the hinge region corresponding to residues 226, 227, 228, 229, and 230 of IGHG4 (Kabat numbering).
Dependencies are inferred from the claim text.