Anti-b7-h3 antibodies and diagnostic uses thereof
AssigneeSpring BioScience Corporation
InventorsFernando Jose Rebelo do Couto, Zhiming Liao, Yifei Zhu
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Abstract
Provided herein are B7-H3 antibodies, fragments of such antibodies, and compositions comprising the same. The antibodies, antibody fragments and compositions are useful in a number of analytical methods, including immunohistochemical and immunocytochemical detection and analysis of B7-H3. Also provided herein are isolated peptides and fusion proteins containing immunogenic determinants for said B7-H3 antibodies, animals immunized with the peptides and fusion proteins, isolated B cells obtained from the animals, and hybridomas made from the isolated B cells.
Claims20
A composition comprising a fragment of human B7-H3 from 14 to 50 amino acids in length linked to a carrier protein, wherein the fragment of human B7-H3 comprises SEQ ID NO:
- 1.
An isolated B cell capable of producing an antibody that specifically binds to SEQ ID NO:
- 1, wherein said isolated B-cell is obtained by a method comprising:
- (a) immunizing an animal with a fragment of human B7-H3 from 14 to 50 amino acids in length linked to a carrier protein, wherein the fragment of human B7-H3 comprises SEQ ID NO:
- 1, and wherein the animal is a rabbit, rat, mouse, or goat;
- and
- (b) isolating the B-cell from the mammal.
A hybridoma capable of producing an antibody that specifically binds to SEQ ID NO:
- 1, wherein the hybridoma is obtained by a method comprising:
- (a) immunizing an animal with a fragment of human B7-H3 from 14 to 50 amino acids in length linked to a carrier protein, wherein the fragment of human B7-H3 comprises SEQ ID NO:
- 1, and wherein the animal is a rabbit, rat, mouse, or goat;
- (b) isolating a B-cell from the mammal, wherein the B-cell produces an antibody that is capable of specifically binding to an epitope of human B7-H3 comprising SEQ ID NO:
- 1;
- and
- (c) preparing the hybridoma from the isolated B-cell.
Dependencies are inferred from the claim text.