Tissue-specific wnt signal enhancing molecules and uses thereof
AssigneeSurrozen, Inc.
InventorsZhengjian ZHANG, Jennifer Jean BRADY, Aaron Ken SATO, Wen-Chen YEH, Yang LI, Teppei YAMAGUCHI
Life of this patent
Estimating the term…
Abstract
The present disclosure provides tissue-specific Wnt signal enhancing molecules, and related methods of using these molecules to increase Wnt signaling in targeted tissues.
Claims55
A tissue-specific Wnt (“Wingless-related integration site” or “Wingless and Int-1” or “Wingless-Int”) signal enhancing molecule, or a pharmaceutically acceptable salt thereof, comprising a first domain that specifically binds one or more transmembrane E3 ubiquitin ligases selected from Zinc and Ring Finger 3 (ZNRF3) and Ring Finger Protein 43 (RNF43), and a second domain that specifically binds a tissue-specific cell surface molecule, wherein the compound increases Wnt signaling in the tissue comprising the tissue-specific cell surface molecule.
A pharmaceutical composition comprising:
- (i) an effective amount of a nucleic acid that encodes a Wnt polypeptide, a Norrin polypeptide, or a Wnt signaling agonist molecule;
- and
- (ii) a pharmaceutically acceptable diluent, adjuvant or carrier.
A method for treating or preventing a disease or condition in a subject in need thereof, wherein the disease or disorder is associated with reduced Wnt (“Wingless-related integration site” or “Wingless and Int-1” or “Wingless-Int”) signaling or would benefit from increased Wnt signaling, comprising providing to the subject a pharmaceutical composition comprising a pharmaceutically acceptable diluent, adjuvant or carrier, and an effective amount of:
- a) a nucleic acid sequence that encodes a Wnt polypeptide, a Norrin polypeptide, or a Wnt signaling agonist molecule, wherein the nucleic acid sequence that encodes the Wnt polypeptide, the Norrin polypeptide, or the Wnt signaling agonist molecule is optionally DNA or mRNA, optionally a modified mRNA;
- b) a vector comprising a nucleic acid sequence that encodes a Wnt polypeptide, a Norrin polypeptide, or a Wnt signaling agonist molecule, wherein the vector comprising the nucleic acid sequence that encodes the Wnt polypeptide, the Norrin polypeptide, or the Wnt signaling agonist molecule is optionally an expression vector;
- or
- c) a host cell comprising an expression vector comprising a nucleic acid that encodes a Wnt polypeptide, a Norrin polypeptide, or a Wnt signaling agonist molecule.
Dependencies are inferred from the claim text.