Method for producing hsl protein having improved catalytic activity for 2-oxoglutaric acid-dependently oxidizing 4-hppd inhibitor

A method for producing a plant with increased resistance to a 4-HPPD inhibitor, comprising the steps of:

• (I) mutating, in an HSL protein of a plant cell, position 140 of an amino acid sequence of SEQ ID NO:
• 4 or an amino acid corresponding to the position to a basic amino acid;
• and
• (II) regenerating a plant from the plant cell in which amino acid mutation is introduced in the step (I).

A method for determining resistance of a plant to a 4-HPPD inhibitor, comprising:

• detecting a nucleotide which codes for position 140 of an amino acid sequence of SEQ ID NO:
• 4 or an amino acid corresponding to the position in an HSL gene of a test plant;
• and
• if the nucleotide codes for a basic amino acid, determining that the test plant has resistance to a 4-HPPD inhibitor.