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AssigneeNanjing Agricultural University
InventorsSHAOLING ZHANG, HUIJUN JIAO, JUYOU WU, LINLIN XU, XIAOSAN HUANG, ZHIHUA XIE, QIAN LIU, YAOJUN CHANG, PENG WANG
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Some embodiments of the disclosure provide a protein of ‘Dangshan Suli’ for promoting the growth of a pollen tube and an encoding gene PbrTTS1. An amino acid sequence of the protein is shown in SEQ ID No.1 of the Sequence Listing. A nucleotide sequence of the encoding gene PbrTTS1 is shown in SEQ ID No.2 of the Sequence Listing. Other embodiments of the disclosure provide a primer for amplifying the encoding gene PbrTTS1. The primer includes a forward primer and a reverse primer. The nucleotide sequence of the forward primer is shown in SEQ ID No. 3 of the Sequence Listing, and the nucleotide sequence of the reverse primer is shown in SEQ ID No. 4 of the Sequence Listing.
A protein of ‘Dangshan Suli’ for promoting a growth of a pollen tube of ‘Dangshan Suli’, wherein an amino acid sequence of the protein is shown in SEQ ID No.1 of the Sequence Listing.
A method for pollination or pollen tube growth, comprising the step of using a protein of ‘Dangshan Suli’, wherein an amino acid sequence of the protein is shown in SEQ ID No.1 of the Sequence Listing.
A method for amplifying an encoding gene PbrTTS1, comprising the steps of:
Dependencies are inferred from the claim text.
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Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from plants
Nucleotides: polynucleotides, e.g. nucleic acids, oligoribonucleotides
For detection or identification of organisms: for plants, fungi or algae
Peptides
Term end not estimated
This application claims priority to Chinese application number 20181064289-2.3, the disclosure of which is incorporated by reference herein in its entirety.
The disclosure relates generally to the field of plant genetic engineering. More specifically, the disclosure relates to the field of a protein of ‘Dangshan Suli’ for promoting the growth of a pollen tube, an encoding gene PbrTTS1 and a use thereof.
A self-incompatibility (SI) phenomenon is a widespread way of controlling inbreeding in the plant kingdom. The SI mainly includes three types, i.e., sporophytic SI, papaveraceae SI, and S-RNase-based gametophytes SI. Among them, the S-RNase-based SI mainly includes species of Rosaceae, Solanaceae and Plantaginaceae (Clarke and Newbigin 1993; Huang et al. 2009).
FIG. 1 is a technical flow chart showing an embodiment of the disclosure.
FIG. 2 is a diagram showing the expression situations of the PbrTTS1 gene in various tissues of the pear.
FIG. 3 is a sequence alignment diagram of the PbrTTS1 in different pear strains.
According to an embodiment, the disclosure may provide a protein of ‘Dangshan Suli’ for promoting the growth of a pollen tube of ‘Dangshan Suli’, and the amino acid sequence of the protein is shown in SEQ ID No.1 of the Sequence Listing.
According to another embodiment, the disclosure provides a method for preparing the protein of ‘Dangshan Suli’ for promoting the growth of the pollen tube of the ‘Dangshan Suli’, including a method for constructing a recombinant prokaryotic expression system and a method for expressing and purifying the recombinant protein.
According to a further embodiment, the disclosure may provide a method for constructing the recombinant prokaryotic expression system may include the following steps.